Portugal Squad
Goalkeepers: Diogo Costa, Jose Sa, Rui Silva
Defenders: Nelson Semedo, Ruben Dias, Tomas Araujo, Diogo Dalot, Renato Veiga, Goncalo Inacio, Joao Cancelo, Nuno Mendes
Midfielders: Matheus Nunes, Bruno Fernandes, Bernardo Silva, Joao Neves, Ruben Neves, Vitinha, Samu Costa
Forwards: Cristiano Ronaldo, Goncalo Ramos, Joao Felix, Francisco Trincao, Rafael Leao, Pedro Neto, Goncalo Guedes, Francisco Conceicao
Portugal boss Roberto Martinez has no major fresh injury concerns reported after the dramatic win against Croatia.
Goncalo Ramos is pushing for a start after scoring the stoppage-time winner in Toronto, but Cristiano Ronaldo should continue to lead the attack.
Four goals were disallowed in the same game for the first time in tournament history. Croatia saw three efforts chalked off, including Josko Gvardiol's 103rd-minute equaliser, while Ronaldo also had a goal ruled out.
“I have incredible admiration for Croatia, and I admire their sporting nature,” Martinez said.
“But the balls now have a chip in them, and it is clear why VAR intervened. The decisions were all correct today; the penalty [for Portugal] was clear.
“It’s a shame that one of the two teams had to lose, but there was no bad decision or lucky call.”
Spain Squad
Goalkeepers: David Raya, Joan Garcia, Unai Simon
Defenders: Marc Pubill, Alex Grimaldo, Eric Garcia, Marcos Llorente, Pedro Porro, Aymeric Laporte, Pau Cubarsi, Marc Cucurella
Midfielders: Mikel Merino, Fabian Ruiz, Gavi, Dani Olmo, Yeremy Pino, Alex Baena, Rodri, Martin Zubimendi, Pedri
Forwards: Ferran Torres, Nico Williams, Lamine Yamal, Mikel Oyarzabal, Victor Munoz, Borja Iglesias
Spain boss Luis de la Fuente is expected to keep faith with the side that cruised past Austria.
La Roja have two injury worries this week, with Yeremy Pino (shoulder) and Nico Williams (adductor), both expected to miss the last-16 clash.
Barcelona star Lamine Yamal will start after an impressive showing against the Austrians.
"I have many reasons to be happy today. First, because of the victory, we won significantly, and the image of the team was fabulous," De la Fuente told reporters.
"But there is still room for improvement. Perhaps you think you have seen the best of us, but this team has not yet reached its capabilities. There is more to do."